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35 cycles, 92C for 1 minute, 60C for 20 seconds, 72C for 30 seconds
Degenerate Primers For Universal Amplification of Diagnostic ~576 - 579 bp Core Coat Protein Gene Fragment:
- Virus
(+) Strand Primer: prV324 5' gcc(ct)at(ga)ta(tc)ag(ag)aagcc(ac)ag3'
- Virus
(- ) Strand Primer: prC889 5' gg(ag)tt(atg)ga(ga)gcatg(tca)gtacatg3'
Electrophoresis
of PCR product in 1.5% agarose gel (TAE, pH 8.0)
Option to clone and sequence, or to directly sequence the product.
Reference: Wyatt, S.D., and J.K. Brown. 1996. Phytopathology 86: 1288-1293.
